免疫细胞培养- ABclonal

免疫细胞培养专用细胞因子

巨噬细胞（Macrophage）通常被认为是由骨髓中的造血干细胞到髓系祖细胞到单核细胞发育而来，迁移到组织后分化为组织特异性巨噬细胞，它存在两个不同的亚群：M1型（经典激活）和M2型（替代激活）。M1型巨噬细胞具有促炎作用，能被脂多糖（LPS）单独或与Th1细胞因子（如IFN-γ，GM-CSF）联合极化，并产生促炎细胞因子，如IL-1β、IL-6、IL-12、IL-23和TNF-α；M2型巨噬细胞具有抗炎和免疫调节作用，能被Th2细胞因子IL-4和IL-13极化，产生IL-10和TGF-β等抗炎细胞因子。M1/M2巨噬细胞的平衡极化对于维系机体健康至关重要。

巨噬细胞培养常用细胞因子收起

蛋白名称	物种	Gene ID	标签	表达系统	货号
Recombinant Human CSF-1/M-CSF Protein	Human	1435	C-His	HEK293 cells	RP01221
Recombinant Mouse CSF-1/M-CSF Protein	Mouse	12977	No-Tag	HEK293 cells	RP01216S
Recombinant Rat CSF-1/M-CSF Protein	Rat	78965	C-His	HEK293 cells	RP01847
Recombinant Human CSF-2/GM-CSF Protein	Human	1437	C-His	HEK293 cells	RP00094
Recombinant Mouse CSF-2/GM-CSF Protein	Mouse	12981	N-His	HEK293 cells	RP01206
Recombinant Rat CSF-2/GM-CSF Protein	Rat	116630	N-His	HEK293 cells	RP01207
Recombinant Human IFN-gamma Protein	Human	3458	No tag	E. coli	RP01038
Recombinant Mouse IFN-gamma Protein	Mouse	15978	C-hFc&His	HEK293 cells	RP01070
Recombinant Rat IFN-gamma Protein	Rat	25712	C-hFc&His	HEK293 cells	RP01075
Recombinant Human IL-4 Protein	Human	3565	C-His	HEK293 cells	RP00995
Recombinant Mouse IL-4 Protein	Mouse	16189	C-His	HEK293 cells	RP01161
Recombinant Rat IL-4 Protein	Rat	287287	C-His	HEK293 cells	RP01874
Recombinant Human IL-13 Protein	Human	3596	C-His	HEK293 cells	RP01320
Recombinant Mouse IL-13 Protein	Mouse	16163	C-His	HEK293 cells	RP01596
Recombinant Human TNF-alpha Protein	Human	7124	C-His	HEK293 cells	RP00993
Recombinant Mouse TNF-alpha Protein	Mouse	21926	C-His	HEK293 cells	RP01071
Recombinant Rat TNF-alpha Protein	Rat	24835	C-His	HEK293 cells	RP01322
Recombinant Human IL-10 Protein	Human	3586	C-His	HEK293 cells	RP00093
Recombinant Mouse IL-10 Protein	Mouse	16153	C-His	HEK293 cells	RP01465
Recombinant Rat IL-10 Protein	Rat	25325	C-His	HEK293 cells	RP03252
LPS (Lipopolysaccharides)	Escherichia coli 055:B5	/	/	用于激活免疫细胞（如体外培养巨噬细胞时诱导细胞因子分泌）等。	RPM0001
PMA (佛波酯) (PKC激活剂)	16561-29-8	/	固体	本产品仅用于科研。1. 细胞生物学研究\|2. 肿瘤研究\|3. 炎症研究	RPM0015

巨噬细胞鉴定常用标志物抗体收起

巨噬细胞类型	物种	标志物
M0	Mouse	F4/80
		CD11b
		CD68
M1	Mouse	CD80
M1	Mouse	CD86
M2	Mouse	CD163
		CD204
		CD206
		CD209

巨噬细胞类型	物种	标志物
M0	Human	F4/80
		CD11b
		CD68
M1	Human	CD80
M1	Human	CD86
M2	Human	CD163
		CD204
		CD206
		CD209

巨噬细胞培养验证数据展示

部分竞品对比数据展示

RP01216S/RP01216

Mouse M-CSF Protein

Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the M-NFS-60 cells.

RP01161

Mouse IL-4 Protein

Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells

RP01465

Mouse IL-10 Protein

Recombinant Mouse IL-10 promote cell proliferation of MC/9 mouse mast cells

客户培养数据展示

浙江大学

Experiment Type: Cell-based assay
Sample: Mouse bone marrow cells
Product: Recombinant Mouse CSF-1/M-CSF Protein (RP01216)
Description: Mouse bone marrow cells were taken and cultured in a cell culturedish for 3 hours. Following this, the cells were transferred to a bacteria culture dish and cultured inmedium containing 20 ng/mL M-CSF, The medium was changed every 3 days, and after 7 days, the cells were matured, Flow cytometrywas employed to assess changes in macrophage markers, revealing a positive rate of macrophages as high as 98%. Additionally, results from phagocytosis experiments indicated that the induced macrophages exhibited strong phagocytic capability.

中国人民解放军陆军军医大学第一附属医院

Experiment Type: Cell-based assay
Sample: Mouse bone marrow cells
Product: Recombinant Mouse CSF-1/M-CSF Protein (RP01216); Recombinant Mouse IL-4 Protein (RP01161)
Description: 1、Extract the bone marrow cells of a 8-week-old male C57 mouse, stimulate the cells with mouse CSF-1 (Cat: RP01216) at a final concentration of 20 ng/mL for one week (change the culture mediumevery day). Collect the cells, and detect the result of macrophage induction by flow cytometry.

2.Stimulate the M0 cells with mouse IL-4 (Cat. RP01161) at a final concentration of 40 ng/mL. Then collect the cells detect the result of M2 polarization by flow cytometry.

操作步骤

细胞因子复溶及保存操作收起

1. 未添加载体蛋白的产品，如RP01216S、RP01161等操作如下：
使用含0.1% BSA或者10% FBS的无菌PBS将重组M-CSF细胞因子复溶至100 μg/mL（10 μg因子加100 μL PBS，100 μg因子加1 mL PBS，以此类推），将复溶后的M-CSF母液按10 μL/管进行分装，冻存在-20℃以下，随取随用，避免反复冻融。
2. 本身已添加了载体蛋白的产品，如RP01216SC、RP01161C操作如下：
使用无菌PBS将重组M-CSF细胞因子复溶至100 μg/mL，无需额外添加保护剂（10 μg因子加100 μL PBS，100 μg因子加1 mL PBS，以此类推），将复溶后的M-CSF母液按10 μL/管进行分装，冻存在-20℃以下，随取随用，避免反复冻融。
3. 含0.1%BSA的PBS配制方法如下：称取10 mg BSA溶解在无菌PBS中，定容至10 mL，用0.22 μm的滤膜过滤除菌后使用。

获取小鼠骨髓细胞收起

1. 取C57BL/6J雄性小鼠（6-8周龄）颈椎脱臼法处死，手术取出所有股骨和胫骨，并用剪刀和镊子将骨周围的肌肉组织尽量去除干净，不要损伤到骨;
2. 将骨移至超净台内，并用盛有70%酒精的无菌培养皿浸泡2-5 min，以消毒灭菌，然后用无菌PBS（含1% P/S）洗2次；
3. 将骨移入另一个盛有PBS（含1% P/S）或RPMI 1640（含1% P/S）的新培养皿中，用剪刀剪去骨两端，再用注射器抽取PBS（含1%P/S）或RPMI 1640（含1% P/S），针头分别从骨两端插入骨髓腔，反复冲洗出骨髓至培养皿中，直至骨完全变白；
4. 收集骨髓细胞悬液，用200目尼龙网滤去小碎片和肌肉组织；
5. 滤过液400g离心5 min，弃上清；
6. 加入5mL 红细胞裂解液吹打混匀，室温裂解5min后，400g离心5 min（如果细胞沉淀仍有较多红细胞，可重复裂解一次）；
7. 使用完全培养基(α-MEM+10%FBS(热灭活)+1% P/S)重悬细胞，即获得小鼠骨髓细胞，一般一只小鼠大约可获得2×10⁷-4×10⁷个骨髓细胞；
注：红细胞裂解时最长不超过10 min,以免对有核细胞造成损伤。

M0巨噬细胞诱导收起

1. 使用完全培养基(α-MEM+10%FBS(热灭活)+1% P/S)将细胞培养过夜，以去除贴壁较快的其他杂细胞如纤维细胞等等；收集未贴壁细胞，调整细胞浓度为2×10⁶-3×10⁶/ mL，铺板培养（10 cm未TC处理的细胞培养皿，每皿10 mL细胞悬液），在完全培养基（α-MEM+10%FBS(灭活)+1%P/S）中添加50 ng/mL M-CSF诱导向巨噬细胞成熟；每隔1天换液一次；
2. 培养4-6天分化为成熟巨噬细胞（贴壁），倒置显微镜观察细胞形态，同时选择F4/80+ CD11b+双阳表达作为鉴定巨噬细胞成熟的标志。
注：（1）血清热灭活处理方法，56℃处理30 min。目的是使血清中的补体失活而不影响其他的有助于细胞生长的成分，避免补体对培养的巨噬细胞不必要的激活或者破坏作用；
（2）巨噬细胞的生长和增殖依赖于M-CSF，如果诱导过程中发现细胞漂了，可能的原因之一是M-CSF耗尽，需要补加因子；
（3）使用未TC处理的培养皿/培养板进行培养，目的是便于将贴壁细胞吹洗下来，避免用胰酶消化影响细胞活率。

M1/M2型巨噬细胞极化收起

1. 将成熟的M0巨噬细胞用无菌PBS轻轻吹洗离心2遍后，重新调整细胞密度5×10⁵/孔（未TC处理的6孔板）并铺板，添加相关细胞因子，诱导其向M1、M2型巨噬细胞的极化：
M1极化因子：IFN-γ（50 ng/mL）+ LPS (100 ng/mL)
M2极化因子：IL-4 (核心因子, 20 ng/mL)，IL-13（可选，20-50 ng/mL）, IL-10（可选，20-50 ng/mL）
2. 刺激24-48 h后，用流式检测M0(F4/80+, CD11b+)和极化后M1(CD86+, CD80+等)、M2(CD206+，Arg-1+等)标志蛋白的表达
对极化巨噬细胞进行形态学观察：巨噬细胞极化后形态发生变化，M1巨噬细胞通常呈现出更圆或不规则的形态,M2巨噬细胞倾向于呈现细长的形态，有时会形成纺锤形的细胞。

巨噬细胞流式鉴定操作收起

1. 将细胞重悬于PBS中，调整细胞浓度至1×107/mL。
2. 每管加入100 μL细胞悬液（约1×106细胞），加入相应的的荧光标记抗体，并设置相应的对照（根据抗体说明书调整），经典鉴定方案如下：
M0：F4/80+，CD11b+
M1：F4/80+，CD11b+，CD80+/CD86+, CD206-
M2：F4/80+，CD11b+，CD80-/CD86-, CD206+
3. 避光孵育15 - 30分钟，4℃。
4. 加入2 mL PBS洗涤细胞，1500 rpm离心5分钟，弃上清。
5. 重复洗涤一次，重悬细胞于200 μL PBS中，上机检测。
注：CD68和CD206为跨膜抗原，需要确定相应的克隆号为识别胞外或者胞内区域，流式染色需要做固定破膜胞内染色。

树突状细胞（Dendritic cell, DC）是一类来源于骨髓造血系统的异质性细胞，可由髓系祖细胞和淋巴祖细胞发育而来，是目前发现的功能最强的抗原呈递细胞（APC）。DC细胞能在天然免疫中识别病原体，在适应性免疫中通过MHC分子呈递抗原肽来诱导Naïve T细胞激活和分化，是先天免疫和适应性免疫之间的主要桥梁。在常规的DC诱导培养中，GM-CSF、IL-4和TNF-α被认为是最重要的三大细胞因子，其中GM-CSF促进DC生成；IL-4通过抑制DC前体细胞向巨噬细胞分化，而分化为未成熟的DC；加入TNF-α后，DC前体细胞即向成熟表型DC分化。

树突状细胞培养常用细胞因子

蛋白名称	物种	Gene ID	标签	表达系统	货号
Recombinant Human CSF-2/GM-CSF Protein	Human	1437	C-His	HEK293 cells	RP00094
Recombinant Mouse CSF-2/GM-CSF Protein	Mouse	12981	N-His	HEK293 cells	RP01206
Recombinant Rat CSF-2/GM-CSF Protein	Rat	116630	N-His	HEK293 cells	RP01207
Recombinant Human Flt3 ligand/Flt3L Protein	Human	2323	C-His	HEK293 cells	RP00175
Recombinant Mouse Flt3 ligand/Flt3L Protein	Mouse	14256	C-hFc&His	HEK293 cells	RP01058
Recombinant Human IL-4 Protein	Human	3565	C-His	HEK293 cells	RP00995
Recombinant Mouse IL-4 Protein	Mouse	16189	C-His	HEK293 cells	RP01161
Recombinant Rat IL-4 Protein	Rat	287287	C-His	HEK293 cells	RP01874
Recombinant Human IL-6 Protein	Human	3569	C-His	HEK293 cells	RP00201
Recombinant Mouse IL-6 Protein	Mouse	16193	C-His	HEK293 cells	RP01321
Recombinant Rat IL-6 Protein	Rat	24498	C-His	HEK293 cells	RP01741
Recombinant Human IL-1 beta Protein	Human	3553	C-His	E. coli	RP00002
Recombinant Mouse IL-1 beta Protein	Mouse	16176	C-His	E. coli	RP01340
Recombinant Rat IL-1 beta Protein	Rat	24494	C-His	HEK293 cells	RP01788
Recombinant Human TNF-alpha Protein	Human	7124	C-His	HEK293 cells	RP00993
Recombinant Mouse TNF-alpha Protein	Mouse	21926	C-His	HEK293 cells	RP01071
Recombinant Rat TNF-alpha Protein	Rat	24835	C-His	HEK293 cells	RP01322
LPS (Lipopolysaccharides)	Escherichia coli 055:B5	/	/	用于激活免疫细胞（如体外培养巨噬细胞时诱导细胞因子分泌）等。	RPM0001

树突状细胞培养验证数据展示

部分竞品对比数据展示

RP01206

Mouse CSF-2/GM-CSF Protein

Recombinant Mouse CSF-2/GM-CSF stimulates cell proliferation of FDC-P1 cells

RP00094

Human CSF-2/GM-CSF Protein

Recombinant Human CSF-2/GM-CSF stimulates cell proliferation of FDC-P1 cells

RP01161

Mouse IL-4 Protein

Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells

客户培养数据展示

厦门大学

Experiment Type: Cell-based assay
Sample: Mouse bone marrow cells
Product: Recombinant Mouse IL-4 Protein (RP01161)
Description: C57BL/6 mouse bone marrow cells were induced with IL-4 (10 ng/mL, Cat. RP01161) and GM-CSF (20 ng/mL) to differentiate into bone marrow derived dendritic cells (BMDC). At 5 days and 6 days after induction, the expression of CD11c and MHC Ⅱ were detected by flow cytometry to analyze the maturation of BMDC.

中山大学

Experiment Type: Cell-based assay
Sample: Mouse bone marrow cells
Product: Recombinant Mouse Flt3 ligand/Flt3L Protein (RP01058)
Description: Recombinant Mouse FLT3L(80 ng/mL) induce C57BL/6 mouse bone marrow cells to differentiate into BMDC. After 12 days, the maturity of dendritic cells was measured by flow cytometry.

自然杀伤细胞（Natural killer cell，NK细胞）来源于骨髓造血干细胞，属于先天免疫系统的核心细胞，由共同淋巴祖细胞发育而来，也是除T细胞和B细胞之外的第三大类淋巴细胞。NK细胞主要分布于外周血、肝脏和脾脏，通过激活和抑制信号的控制来识别和杀死靶细胞。由于具有无MHC限制的自然杀伤活性、通过分泌IFN-γ等细胞因子和趋化因子参与免疫调节、能介导抗体依赖细胞介导的细胞毒性作用（ADCC），NK细胞常被用于开发肿瘤免疫细胞疗法。在体外培养过程中常用IL-2、IL-12、IL-15、IL-21等细胞因子刺激NK细胞，提高其活性。

NK细胞培养常用细胞因子

蛋白名称	物种	Gene ID	标签	表达系统	货号
Recombinant Human IL-2 Protein	Human	3558	C-His	HEK293 cells	RP01039
Recombinant Mouse IL-2 Protein	Mouse	16183	C-His	HEK293 cells	RP01384
Recombinant Rat IL-2 Protein	Rat	116562	C-His	HEK293 cells	RP01769
Recombinant Human IL-7 Protein	Human	3574	C-His	HEK293 cells	RP01040
Recombinant Mouse IL-7 Protein	Mouse	16196	C-6His	HEK293 cells	RP01766
Recombinant Rat IL-7 Protein	Rat	25647	C-6His	HEK293 cells	RP01721
Recombinant Human IL-12/IL-12A&IL-12B Protein	Human	3592&3593	C-His(IL12B)&C-Flag(IL12A)	HEK293 cells	RP01232
Recombinant Mouse IL-12/IL-12A&IL-12B Protein	Mouse	16159&16160	C-His(IL-12a)&No tag(IL-12b)	HEK293 cells	RP01691
Recombinant Human IL-15 Protein	Human	3600	C-His	E. coli	RP01236
Recombinant Mouse IL-15 Protein	Mouse	16168	C-6His	E. coli	RP01676
Recombinant Human IL-18 Protein	Human	3606	No tag	E. coli	RP00006
Recombinant Mouse IL-18 Protein	Mouse	16173	C-His	HEK293 cells	RP02521
Recombinant Mouse IL-21 Protein	Mouse	60505	C-His	CHO Cells	RP02775S
Recombinant Rat IL-21 Protein	Rat	365769	C-6His	HEK293 cells	RP01756

NK细胞培养验证数据展示

部分竞品对比数据展示

RP01039

Human IL-2 Protein

Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.

RP01384

Mouse IL-2 Protein

Recombinant Mouse IL-2 stimulates cell proliferation of the CTLL2 cells

RP01766

Mouse IL-7 Protein

Recombinant Mouse IL-7 stimulates cell proliferation of the murine 2E8 cells

客户培养数据展示

南京中医药大学

Experiment Type: Cell-based assay
Sample: NK92
Product: Recombinant Human IL-2 Protein (RP01039)
Description: After NK92 cells were stimulated with 100 ng/mL IL-2 for 48 h, the number of clusters increased significantly and the activity was enhanced.

T细胞是适应性免疫能力的重要组成部分，可对病原体启动细胞介导的免疫应答，大致分为辅助性T细胞（CD4+T细胞）和细胞毒性T细胞（CD8+T细胞）。CD4+T细胞包含Th1、Th2、Th9、Th17、Th22、Treg和Tfh等不同的亚群，通过分泌细胞因子产生炎症或激活其他免疫细胞。CD8+T细胞按照分泌的细胞因子的不同分为Tc1，Tc2，Tc17, Tc9和Tc22等亚群，可直接杀死被病原体感染或癌变的细胞。每种T细胞亚群都由一组特定的细胞因子和转录因子活化，这些细胞因子在T细胞增殖、分化的培养过程中发挥了重要作用。

T细胞培养常用细胞因子

蛋白名称	物种	Gene ID	标签	表达系统	货号
Recombinant Rat IL-1 alpha Protein	Rat	24493	C-6His	HEK293 cells	RP01736
Recombinant Human IL-1 beta Protein	Human	3553	C-His	E. coli	RP00002
Recombinant Mouse IL-1 beta Protein	Mouse	16176	C-His	E. coli	RP01340
Recombinant Rat IL-1 beta Protein	Rat	24494	C-His	HEK293 cells	RP01788
Recombinant Human IL-2 Protein	Human	3558	C-His	HEK293 cells	RP01039
Recombinant Mouse IL-2 Protein	Mouse	16183	C-His	HEK293 cells	RP01384
Recombinant Rat IL-2 Protein	Rat	116562	C-His	HEK293 cells	RP01769
Recombinant Human IL-4 Protein	Human	3565	C-His	HEK293 cells	RP00995
Recombinant Mouse IL-4 Protein	Mouse	16189	C-His	HEK293 cells	RP01161
Recombinant Rat IL-4 Protein	Rat	287287	C-His	HEK293 cells	RP01874
Recombinant Human IL-6 Protein	Human	3569	C-His	HEK293 cells	RP00201
Recombinant Mouse IL-6 Protein	Mouse	16193	C-His	HEK293 cells	RP01321
Recombinant Rat IL-6 Protein	Rat	24498	C-His	HEK293 cells	RP01741
Recombinant Human IL-7 Protein	Human	3574	C-His	HEK293 cells	RP01040
Recombinant Mouse IL-7 Protein	Mouse	16196	C-6His	HEK293 cells	RP01766
Recombinant Rat IL-7 Protein	Rat	25647	C-6His	HEK293 cells	RP01721
Recombinant Human IL-12/IL-12A&IL-12B Protein	Human	3592&3593	C-His(IL12B)&C-Flag(IL12A)	HEK293 cells	RP01232
Recombinant Mouse IL-12/IL-12A&IL-12B Protein	Mouse	16159&16160	C-His(IL-12a)&No tag(IL-12b)	HEK293 cells	RP01691
Recombinant Human IL-15 Protein	Human	3600	C-His	E. coli	RP01236
Recombinant Mouse IL-15 Protein	Mouse	16168	C-6His	E. coli	RP01676
Recombinant Mouse IL-21 Protein	Mouse	60505	C-His	CHO Cells	RP02775S
Recombinant Rat IL-21 Protein	Rat	365769	C-6His	HEK293 cells	RP01756
Recombinant Human IL-23/IL-12B&IL-23A Protein	Human	3593&51561	No tag(IL12B)&N-his(IL23A)	HEK293 cells	RP01160
Recombinant Mouse IL-23/IL-12B&IL-23A Protein	Mouse	16160&83430	N-His(IL-23a)&No tag(IL-12b)	HEK293 cells	RP02928S
Recombinant Cynomolgus IL-23A&Mouse IL-12B Protein	Cynomolgus&Mouse	16160&102128555	N-His	HEK293 cells	RP02725
Recombinant Human IL-33 Protein	Human	90865	No tag	E. coli	RP00019
Recombinant Mouse IL-33 Protein	Mouse	77125	No tag	E. coli	RP00618
Recombinant Human IFN-gamma Protein	Human	3458	No tag	E. coli	RP01038
Recombinant Mouse IFN-gamma Protein	Mouse	15978	C-hFc&His	HEK293 cells	RP01070
Recombinant Rat IFN-gamma Protein	Rat	25712	C-hFc&His	HEK293 cells	RP01075
Recombinant Human TNF-alpha Protein	Human	7124	C-His	HEK293 cells	RP00993
Recombinant Mouse TNF-alpha Protein	Mouse	21926	C-His	HEK293 cells	RP01071
Recombinant Rat TNF-alpha Protein	Rat	24835	C-His	HEK293 cells	RP01322
Recombinant Human Mature TGF-beta 1 Protein	Human	7040	No tag	HEK293 cells	RP01458
Recombinant Mouse/Rat Mature TGF-beta 1 Protein	Mouse	21803	No tag	HEK293 cells	RP00671
Recombinant Human CXCL13/BLC Protein	Human	10563	No tag	E. coli	RP02839

T细胞培养验证数据展示

部分竞品对比数据展示

RP01039

Human IL-2 Protein

Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.

RP01384

Mouse IL-2 Protein

Recombinant Mouse IL-2 stimulates cell proliferation of the CTLL2 cells

RP01691

Mouse IL-12 Protein

Recombinant Mouse IL-12 Protein induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells

客户培养数据展示

厦门大学

Experiment Type: Cell-based assay
Sample: T cell
Product: Recombinant Mouse IL-12/IL-12A&IL-12B Protein (RP01691)
Recombinant Human Mature TGF-beta 1 Protein (RP01458)
Recombinant Mouse IL-23/IL-12B&IL-23A Protein (RP02928S)（Upgraded product）
Description: A.Recombinant mouse IL-12 (Cat. RP01691) induced mouse Th1 differentiation. The expression of IFN-γ was detected by flow cytometry.
B.Recombinant mouse IL-23 (Cat. RP02928LQ) and human TGF-β (Cat. RP01458) induced mouse Th17 differentiation. The expression of IL-17α was detected by flow cytometry.

中山大学

Experiment Type: Cell-based assay
Sample: Memory T cell
Product: Recombinant Human IL-2 Protein (RP01039)
Recombinant Human IL-7 Protein (RP01040)
Recombinant Human IL-15 Protein (RP01236)
Description: Human Memory T cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), IL-7 (10 ng/mL, Cat. RP01040) and IL-15 (10 ng/mL, Cat. RP01236), and the cells were observed at 0h, 24h, 36h, 48h. T Cell growth was good and cell proliferation was obvious.

B细胞是一种能够对病原体启动抗体介导的免疫应答的适应性免疫细胞，可生成高亲和力抗体、形成免疫记忆、充当抗原呈递细胞，并分泌细胞因子。骨髓中的B细胞源自造血干细胞，由其分化为共同淋巴祖细胞，随之生成自然杀伤细胞、T淋巴细胞和B淋巴细胞。在B细胞的发育过程中，有多种细胞因子会影响其生长、存活和抗体类型转换，例如IFN-α和IFN-β主要参与B细胞的生成和选择，而IL-4和IL-6则影响B细胞优先分泌特定类别的抗体。

B细胞培养常用细胞因子

蛋白名称	物种	Gene ID	标签	表达系统	货号
Recombinant Human IL-2 Protein	Human	3558	C-His	HEK293 cells	RP01039
Recombinant Mouse IL-2 Protein	Mouse	16183	C-His	HEK293 cells	RP01384
Recombinant Rat IL-2 Protein	Rat	116562	C-His	HEK293 cells	RP01769
Recombinant Human IL-4 Protein	Human	3565	C-His	HEK293 cells	RP00995
Recombinant Mouse IL-4 Protein	Mouse	16189	C-His	HEK293 cells	RP01161
Recombinant Rat IL-4 Protein	Rat	287287	C-His	HEK293 cells	RP01874
Recombinant Human IL-6 Protein	Human	3569	C-His	HEK293 cells	RP00201
Recombinant Mouse IL-6 Protein	Mouse	16193	C-His	HEK293 cells	RP01321
Recombinant Rat IL-6 Protein	Rat	24498	C-His	HEK293 cells	RP01741
Recombinant Human IL-7 Protein	Human	3574	C-His	HEK293 cells	RP01040
Recombinant Mouse IL-7 Protein	Mouse	16196	C-6His	HEK293 cells	RP01766
Recombinant Rat IL-7 Protein	Rat	25647	C-6His	HEK293 cells	RP01721
Recombinant Human IL-10 Protein	Human	3586	C-His	HEK293 cells	RP00093
Recombinant Mouse IL-10 Protein	Mouse	16153	C-His	HEK293 cells	RP01465
Recombinant Rat IL-10 Protein	Rat	25325	C-His	HEK293 cells	RP03252
Recombinant Mouse IL-21 Protein	Mouse	60505	C-His	CHO Cells	RP02775S
Recombinant Rat IL-21 Protein	Rat	365769	C-6His	HEK293 cells	RP01756
Recombinant Human c-Kit ligand/KITLG/SCF Protein	Human	4254	C-His	HEK293 cells	RP00124
Recombinant Mouse c-Kit ligand/KITLG/SCF Protein	Mouse	17311	C-His	HEK293 cells	RP01055
Recombinant Rat c-Kit ligand/KITLG/SCF Protein	Rat	60427	C-His	HEK293 cells	RP01794
Recombinant Human TNFSF11/RANKL/CD254 Protein	Human	8600	N-His	HEK293 cells	RP00183
Recombinant Mouse TNFSF11/RANKL/CD254 Protein	Mouse	21943	N-His＆N-hFC	HEK293 cells	RP02134
Recombinant Human TNFSF5/CD40 ligand/CD154 Protein	Human	959	N-6His	HEK293 cells	RP01733
Recombinant Mouse TNFSF5/CD40 ligand/CD154 Protein	Mouse	21947	N-6His	HEK293 cells	RP01734
Recombinant Human TNFSF13B/BAFF/CD257 Protein	Human	10673	No tag	E. coli	RP00018

B细胞培养验证数据展示

部分竞品对比数据展示

RP01039

Human IL-2 Protein

Recombinant Human IL-2 stimulates cell proliferation of the CTLL2 cells.

RP01161

Mouse IL-4 Protein

Recombinant Mouse IL-4 stimulates cell proliferation of the HT-2 cells

RP01766

Mouse IL-7 Protein

Recombinant Mouse IL-7 stimulates cell proliferation of the murine 2E8 cells

客户培养数据展示

复旦大学附属中山医院

Experiment Type: Cell-based assay
Sample: Primary B cell
Product: Recombinant Human IL-2 Protein (RP01039)
Recombinant Human TNFSF13B/BAFF/CD257 Protein (RP00018)
Description: Human primary B cells were cultured with IL-2 (20 ng/mL, Cat. RP01039), BAFF (10 ng/mL, Cat. RP00018), IL-21 (20 ng/mL, Cat. RP02508), and the cells grew well and formed into clusters after activation.

厦门大学

Experiment Type: Cell-based assay
Sample: B cell
Product: Recombinant Mouse IL-4 Protein (RP01161)
Description: B cells were cultured using mouse IL-4 (1 ng/mL, Cat. RP01161) for 4 days. Obvious cluster-like growing cells could be observed, indicating that IL-4 provides the necessary signals for the proliferation and activation of B cells.