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Phospho-CDK2-T160 Rabbit mAb (AP1364)

Western blot analysis of lysates from HeLa cells, using Phospho-CDK2-T160 Rabbit mAb (AP1364) at 1:5000 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.

Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit mAb (AP1364) at 1:5000 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-CDK2-T160 Rabbit mAb (AP1364) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of paraffin-embedded human placenta using Phospho-CDK2-T160 Rabbit mAb (AP1364) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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货号: AP1364

促销价:   ¥1400
货    期:现货产品
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详细信息

推荐稀释比
  • WB1:2000 - 1:6000
  • IHC-P1:50 - 1:200
  • IF/ICC1:50 - 1:200
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请输入产品标签上的lot号,例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
34kDa
实际分子量
34kDa
克隆号
ARC57167
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
阳性样本
HeLa treated by nocodazole,C6 treated by nocodazole
细胞定位
Cajal body,Cytoplasm,Endosome,Nucleus,centrosome,cytoskeleton,microtubule organizing center.
纯化方式
Affinity purification

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背景信息

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

基因ID
Swiss Prot
别名
CDKN2; p33(CDK2); Phospho-CDK2-T160

抗原信息

免疫原
A synthetic phosphorylated peptide around T160 of human CDK2 (NP_001789.2).
序列
TYTHE

关联靶点

CDK2为关键词搜索文献记录,得到上述基因与CDK2的研究紧密相关,排序顺序根据研究相关程度由高到低决定。(所显示数字代表两者共同出现的已发表文献数量)
1/

关联领域

CDK2为关键词搜索文献记录,得到上述相关热词信息,可反映出CDK2的研究趋势与热点。(关联领域与基因名称的距离代表其研究热度)

* 该数据来源于赛特新思(citexs.com)
来自于发表文献,仅供参考所用,ABclonal不承担任何责任。

暂无以CDK2为关键词搜索的文献记录。

该服务由赛特新思提供,上述关联分析来自已发表文献,仅供参考所用,ABclonal不承担任何责任。

ABclonal公司的抗体,可以回收利用几次?

首先,一般抗体不推荐客户回收利用,抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,ABclonal公司也做过一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定。
注:我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存,效价高的抗体可至少保存1周,至少重复利用3次。

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ABclonal作为国产抗体品牌的优势?

武汉爱博泰克生物(ABclonal)科技有限公司是国产品牌,她成立于2011年,公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心,凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士,组成我们的科学家团队,通过聚焦抗体与酶核心技术,致力于打破国际技术的垄断,将公司打造成为科研工具和诊断原料的国内领导品牌,乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台,同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台,我们对每一支自产的抗体进行了严格的检测。当然,我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势:1,严自检,保质量;2产品多,指标全;3,价格低,货期短。注:ABclonal抗体价格体系详情见附件

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ABclonal抗体成分?

ABclonal抗体成分在平时工作当中,常会有客户咨询我们的抗体用的什么buffer进行保存,一般来说,我们的buffer的成分是:PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油;也有一些是PBS含0.03%的proclin300,50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮(MCI)和5-氯-2-甲基-4-异噻唑啉-3-酮(CMCI)。ProClin生物灭活剂能够迅速穿透细胞膜,抑制对细胞呼吸至关重要的特定酶,因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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