产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

NFKBIA Knockout HeLa Cell Line, Homozygous (RM01954)

Genome sequence analysis of PCR products from parental (WT) and NFKBIA knockout (KO) HeLa cells, using sanger sequencing.

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货号: RM01954
促销价:   ¥10000
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详细信息

靶点
NFKBIA
细胞系
HeLa
突变描述
NFKBIA Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:77bp deletion in exon1
Allele-2:77bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell line and 1 vial knockout cell line
细胞数目
1~5x106 cells/vial
使用方法
Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO2 condition.
1. Thaw the vial in 37℃ water bath ,and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
    dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO2.
7. A subcultivation ratio of 1:2-1:4 is recommended.
物种
Human
保存条件
Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
运输条件
Dry ice

背景信息

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

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