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活性验证

Recombinant Human HER1/ERBB1/EGFR (668-1210) Protein (RP02943LQ)

Recombinant Human EGFR (aa668-1210) Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 90-100 kDa.

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货号: RP02943LQ
促销价:   ¥3200
货    期:现货产品
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详细信息

种属
Human
表达宿主
Baculovirus-Insect Cells
Calculated MW
89.1 kDa
Observed MW
89 kDa
标签
N-His&GST
纯度
> 75% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as a 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
描述
Recombinant Human ERBB1/HER1/EGFR (668-1210) Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met668-Ala1210) of Human ERBB1/HER1/EGFR (668-1210) (Accession #NP_005219.2.) fused with  an 6×His, GST tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 0.5 μg/mL (100 μL/well) can bind Rrecombinant human EGFR with a linear range of 0.3-2.5 μg/mL.

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背景信息

The EGFR (epidermal growth factor receptor) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc.The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24?aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain. EGFR signaling has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently, which is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs.

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