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活性验证

Recombinant Mouse GDF15 Protein (RP03177)

Recombinant Mouse GDF15 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-50 kDa.

The purity of Mouse GDF15 is greater than 95% as determined by SEC-HPLC.

Immobilized Mouse GFRAL, His Tag at 2 μg/mL (100μL/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/mL determined by ELISA.

All(3)|
货号: RP03177

促销价:   ¥3200
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
37.9 kDa
Observed MW
40-50 kDa
标签
N-hFc
纯度
> 95% as determined by Tris-Bis PAGE
内毒素
Less than 1EU per μg by the LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Mouse GDF15 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ser189-Ala303) of mouse GDF15 (Accession #Q9Z0J7) fused with a hFc tag at the N-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Immobilized Mouse GFRAL, His Tag at 2 μg/mL (100μL/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/mL determined by ELISA.
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

基因ID
Swiss Prot
别名
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta
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