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Rabbit anti-Human TNF-alpha mAb(DET) (RM17658)

This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.

No significant cross-reactivity or interference was observed.

No significant cross-reactivity or interference was observed.

Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.

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货号: RM17658
促销价:   ¥2100
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详细信息

产品名称
Rabbit anti-Human TNF-alpha mAb(DET)
货号
RM17658
免疫原
Recombinant TNF-alpha Protein
纯化方式
Affinity purification
同种型
Rabbit IgG
内毒素水平
< 1.0 EU/μg of the protein by LAL method.
存储溶液
Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1,PH 7.4.
CrossReactivity
No cross-reactivity in ELISA assay with recombinant
hNFKB1 、mTNFA、rTNFA
存储条件
This antibody can be stored at 2℃-8℃ for one monthwithout detectable loss of activity. Antibody products are stable for twelve months fromdate of receipt when stored at -20℃ to -80℃. Preservative-Free.Avoid repeated freeze-thaw cycles.

* This package insert must be read in its entirety before using this product.

应用

Human TNF-alpha Sanwich ELISA Immunoassay

 Recommended
Concentration
Sample
ELISA Capture0.5-2ug/mLRabbit anti-Human TNF-alpha(CAP)(Cat. No.RM17657)
ELISA Detection0.006-0.025ug/mLRabbit anti-Human TNF-alpha(DET)(Cat. No.RM17658)
Standard7.81-500pg/mLRecombinant Human TNF-alpha Protein

背景信息

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer’s disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

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