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Biotinylated Recombinant Human Latent TGF-beta 1(C33S) Protein (RP02605)

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Biotinylated Human Latent TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Latent TGF beta 1 is greater than 93% as determined by SEC-HPLC.

Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.10μg/ml determined by ELISA.

Immobilized Human ITGAV&ITGB8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39μg/mL determined by ELISA.

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货号: RP02605
促销价：   ￥6000
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.；It contains Leu30-Ser390(C33S).
标签
N-His&Avi
纯度
> 95% as determined by Tris-Bis PAGE；> 93% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein . A furin?like convertase processes the proprotein to generate an N?terminal 249 aa latency?associated peptide (LAP) and a C?terminal 112 aa mature TGF? beta 1. Disulfide?linked homodimers of LAP and TGF? beta 1 remain non?covalently associated after secretion, forming the small latent TGF? beta 1 complex .

基因ID
7040
Swiss Prot
P01137
别名
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
研究领域
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