Proteasome Activity Fluorometric Assay Kit I is designed for assaying the proteasome’s chymotrypsin-like activity in vitro using purified proteasome, cleared cell lysates or tissue extracts. The proteasome cleaves SUC-LLVY-AMC and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.