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Recombinant Human IgG2A Protein (RPT0005)

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Recombinant Human IgG2A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-35 kDa.

Immobilized Human CD32a (Catalog: RP00081) at 2 μg/mL (100 μL/well) can bind Human IgG2 (Catalog: RPT0005) with a linear range of 1.2-338 ng/mL.

All(2)|
货号: RPT0005
促销价：   ￥600
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human IgG2A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu99-Lys326) of human IgG2A (Accession #) fused with and NO-tag at the C-terminus.
标签
NO-tag
纯度
> 97% by SDS-PAGE.
内毒素
<0.1EU/μg
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Human CD32a (Catalog: RP00081) at 2 μg/mL (100 μL/well) can bind Human IgG2 (Catalog: RPT0005) with a linear range of 1.2-338 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens

基因ID
3501
Swiss Prot
P01859
别名
IGHG2;Immunoglobulin heavy constant gamma 2;IgG2
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