![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_WB_01.jpg?t=1704935955)
Western blot analysis of lysates from A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_WB_02.jpg?t=1704935955)
Western blot analysis of various lysates using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_WB_03.jpg?t=1704935955)
Western blot analysis of various lysates using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_01.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded human colon carcinoma tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_02.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded human liver cancer tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_03.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded human placenta tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_04.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded human thyroid cancer tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_05.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded mouse liver tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IHC_06.jpg?t=1704935955)
Immunohistochemistry analysis of Integrin alpha 5 (ITGA5/CD49e) in paraffin-embedded rat liver tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IF_02.jpg?t=1704935955)
Confocal imaging of paraffin-embedded Rat large intestine using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_IF_01.jpg?t=1704935955)
Confocal imaging of paraffin-embedded Human placenta using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A19069, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_FC_01.jpg?t=1704935955)
Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb(A19069, 5 μg/mL,green line) or Rabbit IgG isotype control (AC042, 5 μg/mL,blue line),followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
![](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A19069_ARC0370_FC_02.jpg?t=1704935955)
Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb(A19069, 5 μg/mL,green line) or Rabbit IgG isotype control (AC042, 5 μg/mL,blue line),followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).