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细胞外囊泡(Extracellular Vesicle, EV),包括外泌体,微囊泡、微粒、凋亡小体和其他EV亚群,目前在技术上获得一个不含非囊泡成分的纯EV组分用于功能性研究是具有挑战性的。《MISEV 2018》更新了囊泡研究所需最小的生化、生物物理和功能标准文件[1],用于对细胞外囊泡及其内含物的鉴定和功能分析进行规范。关于外泌体表征鉴定,需包含如下方面:

1. 外泌体通用标志物分析

1)至少包含3个EVs阳性蛋白标志物鉴定
—跨膜或脂质结合蛋白(如CD9,CD63,CD81等)
—胞质蛋白(TSG101,Alix等)
2)至少1个EVs阴性蛋白标志物鉴定(根据样本类型选择)

2. 个体表征:采用以下两种互为补充的方法进行鉴定

1)纳米颗粒跟踪分析(NTA)或纳米流式

ABclonal外泌体表征鉴定相关通用标志物抗体产品

候选靶点[2-3] 产品货号 产品名称 产品应用 反应种属
HSPA8/Hsc70 A0415 Hsc70/HSPA8 Rabbit mAb WB Human, Mouse, Rat
A14001 Hsc70/HSPA8 Rabbit pAb WB, IF Human, Mouse, Rat
A2487 Hsc70/HSPA8 Rabbit pAb WB, IHC,IF Human, Mouse, Rat
HSP90AA1/Hsp90α A5006 Hsp90α Rabbit mAb WB Human, Mouse, Rat
A0365 Hsp90α Rabbit pAb WB, IF Human, Mouse, Rat
HSP90AB1/Hsp90β A19574 Hsp90β Rabbit mAb WB, IF Human, Mouse, Rat
A18015 [KO Validated] Hsp90β Rabbit pAb WB, IHC, IF Human, Mouse, Rat
CD9 A19027 CD9 Rabbit mAb WB, IHC Human, Mouse
A1703 CD9 Rabbit pAb WB, IHC Human, Mouse, Rat
TSG101 A5789 TSG101/VPS23 Rabbit mAb WB Human, Rat
ALIX A2215 ALIX / PDCD6IP Rabbit pAb WB, IHC, IF Human, Mouse, Rat
CD63 A19023 CD63 Rabbit mAb WB Human
A5271 CD63 Rabbit pAb WB, IHC Human, Mouse, Rat
CD81 A4863 CD81 Rabbit mAb WB Mouse, Rat
A5270 CD81 Rabbit pAb WB, IHC,IF Human, Mouse, Rat
FLOT2 A6590 FLOT2 Rabbit pAb WB, IF Human, Mouse, Rat
FLOT1 A6220 Flotillin 1 Rabbit pAb WB, IHC, IF, IP Human, Mouse, Rat
A3023 Flotillin 1 Rabbit mAb WB, IHC Human, Mouse, Rat
HSPA4/Hsp70 A19705 HSPA4 Rabbit mAb WB,IHC Human,Mouse,Rat
ACTB AC026 β-Actin Rabbit mAb (High Dilution) WB, IHC Human, Mouse, Rat,more
AC038 ACTB Rabbit mAb WB, IHC Human, Mouse, Rat,more
RAP1B A12925 RAP1B Rabbit pAb WB, IF Human, Mouse
Moesin A4915 Moesin Rabbit mAb WB, IHC Human, Mouse, Rat
A2M A9752 Alpha-2-Macroglobulin (A2M) Rabbit mAb WB,IHC,IF Human,Mouse,Rat
A1573 Alpha-2-Macroglobulin (A2M) Rabbit pAb WB,IF Human,Mouse,Rat
Gelsolin A2612 Gelsolin Rabbit mAb WB,IHC Human,Mouse
A16869 GSN Rabbit pAb WB,IHC,IF Human,Mouse,Rat
B2M A11642 beta 2 Microglobulin Rabbit mAb WB,IHC Human,Mouse,Rat
A12404 [KO Validated] beta 2 Microglobulin Rabbit pAb WB,IHC,IP Human,Mouse,Rat
A1562 beta 2 Microglobulin Rabbit pAb WB,IHC,IF Human,Mouse,Rat
PRDX2 A4308 Peroxiredoxin 2 (PRDX2) Rabbit mAb WB Human,Mouse,Rat
A1919 [KO Validated] Peroxiredoxin 2 (PRDX2) Rabbit pAb WB,IF Human,Mouse
Filamin A A0927 FLNA Rabbit pAb WB,IF,IP Human,Mouse
A16376 FLNA Rabbit pAb WB,IHC,IF Human,Mouse,Rat
A3738 Filamin A Rabbit mAb WB,IHC Human,Mouse,Rat
A7896 FLNA Rabbit pAb WB,IHC Human,Mouse,Rat
Syntenin A5497 Syntenin Rabbit mAb WB, IHC, IF Human, Mouse, Rat
注:本表格选择的外泌体通用标志物靶点清单来自于文献 Cell. 2020,182(4):1044-1061.e18.  Nat Cell Biol 2021 Jun;23(6)
更多产品查询,请访问ABclonal官网:https://abclonal.com.cn/catalog-antibodies

参考文献

  • 1. J Extracell Vesicles. 2018, 7(1):1535750.
  • 2. Cell. 2020,182(4):1044-1061.e18.
  • 3. Nat Cell Biol 2021 Jun;23(6)