产品和服务 / 产品类型 / 抗体产品 / 标签和内参抗体

重组兔单抗

β-Actin Rabbit mAb (High Dilution) (AC026)

文献引用 (2220) 评论 (6) 说明书 收藏

Western blot analysis of various lysates using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:100000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:10000-1:2560000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

All(5)|
货号: AC026

促销价：   ￥840
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

推荐稀释比
  • WB1:80000-1:640000
  • IHC-P1:100 - 1:500
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat, Chicken, Zebrafish, Pig
同种型
IgG
理论分子量
42kDa
实际分子量
42kDa
克隆号
ARC5115-01
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
阳性样本
TE-1,SW480,NIH/3T3,293T,Mouse brain,Mouse testis,mose lung,rat brain
细胞定位
Cytoplasm,cytoskeleton.
纯化方式
Affinity purification

展开

用户验证的应用

应用及物种
  • WB (rat brain cells , Pig lung , 293T , DF-1Homo sapiens , Danio rerio , Homo sapiens , Mus musculus , Bombyx mori Linnaeus , Homo sapiens , Mus musculus , Oryza sativa , Cricetulus migratorius , Rattus norvegicus , Sus scrofa , Drosophila melanogaster , Gallus gallus , Other , Locusta migratoria , Ctenopharyngodon idellus , Cyprinus carpio , Helicoverpa armigera , Zea mays , Rattus , Solanum lycopersicum , Lotus japonicus , Vaccinium dunalianum , Chlorocebus aethiops , Felis catus , Plutella xylostella , Escherichia coli , Anatinae , Oryctolagus cuniculus , Ziziphus jujuba , Takifugu rubripes , Arabidopsis thaliana , Nicotiana tabacum , Mus , Nicotiana benthamiana , Pelteobagrus fulvidraco , Mus musculus , Rattus norvegicus , Ctenopharyngodon idella , Cotton bollworms , King Charles Spaniel , Pimephales promelas , Edwardsiella tarda , Micropterus salmoides , Oreochromis mossambicus , Chlorocebus aethiops , Bos taurus , Mancinella armigera , Macaca mulatta , Puerariae Flos , Rattus norvegicus , Ovis aries , Cyprinus Carpio , Scylla , turtle , Mauremys sinensis , Mytilus coruscus , juvenile grass , Mesocricetus auratus , Trichopodus trichopterus , Eleusine indica , Brassica napus , Ictalurus punctatus , Haliotis , Eospalax cansus , Bos taurus , Anser cygnoides domesticus , Spondyliosoma cantharus , Fathead minnow , Oreochromis niloticus , Oreochroms mossambcus , Ctenopharyngodon , Meretrix petechialis , Mycobacterium tuberculosis , Mactra , Capra hircus , Dianella ensifolia , Malus micromalus , Pampus argenteus , Cavia porcellus , Echinococcus multilocularis , Anser albifrons , Cricetinae , Hordeum vulgare , Squaliobarbus curriculus , Megalobrama amblycephala , Carassius auratus gibelio , Cynoglossus semilaevis , Alpinia katsumadai Hayata , Acanthopagrus schlegelii , Mus musculusRattus norvegicus , Sus scrofa , Caenorhabditis elegans)
  • IP (Homo sapiens , Mus musculus , Other , Rattus norvegicus , Danio rerio)
  • Co-IP (Homo sapiens , Mus musculus , Zea mays , Other)
  • IF (Mus musculus , Homo sapiens , Sus scrofa , Homo sapiens , Bos taurus , Rattus norvegicus , Danio rerio)
  • Other (Zea mays , Mus musculus , Oryctolagus cuniculus)
  • Enzyme assay (Danio rerio)
  • IHC (Mus musculus , Homo sapiens , Homo sapiens , Mus musculus , Oryctolagus cuniculus , Chlorocebus aethiops , Rattus norvegicus)
  • qPCR (Homo sapiens , Gallus gallus , Cyprinus carpio , Mus musculus)
  • FC (Sus scrofa)
  • RNA-Seq (Mus musculus)
  • RT-qPCR (Mus musculus)
  • qRT-PCR (Gallus gallus)
  • PCR (Rattus norvegicus)
  • RT-PCR (Homo sapiens)
  • ELISA (Rattus norvegicus , Mus musculus)
  • DB (Mus musculus)
  • ChIP (Other)

客户数据及评论 (6)

折叠内容
CustomerJun 032024
评分
Experiment Type
WB
Sample
BV2 cells
Description
Western blot analysis of lysates from mouse BV2 cells using β-Actin Rabbit mAb (AC026) at 1:1000 dilution.
CustomerAug 142022
评分
Experiment Type
WB
Sample
HeLa treated by Starvatio and IGF
Description
Western blot analysis of human cells using β-Actin Rabbit mAb (AC026) at 1:50000 dilution. The band observed at 42KDa.
CustomerAug 302018
评分
Experiment Type
Western blot (WB)
Sample
rat brain cells
Description
Western blot analysis of extracts of rat brain cells, using antibody at 1:50 dilution.
CustomerNov 042016
评分
Experiment Type
Western blot(WB)
Sample
Pig lung
Description
Western blot analysis of extracts of Pig lung, using β-actin Monoclonal Antibody.
CustomerJul 092016
评分
Experiment Type
Western blot(WB)
Sample
293T
Description
Western blot analysis of extracts of 293T cells, using β-actin Monoclonal Antibody.
CustomerJul 082016
评分
Experiment Type
Western blot(WB)
Sample
DF-1
Description
Western blot analysis of extracts of DF-1 cells, using β-actin Monoclonal Antibody.

文献引用 (2220)

折叠内容

Publishing research using AC026? Please let us know so that we can cite the reference in this datasheet.

背景信息

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C.

RRID
AB_2768234
基因ID
60
Swiss Prot
P60709
别名
BRWS1; PS1TP5BP1; β-Actin

抗原信息

免疫原
Recombinant protein of human β-actin
序列
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
进行序列比对
研究领域

关联靶点

以ACTB为关键词搜索文献记录，得到上述基因与ACTB的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以ACTB为关键词搜索文献记录，得到上述相关热词信息，可反映出ACTB的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以ACTB为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

折叠内容

ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

展开

ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

展开

ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

展开

实验方案

折叠内容
>