概述
货号: AC026
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - ACTB Monoclonal Antibody (AC026)
ABclonal:Western blot - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC026)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC026)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC026)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC026)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC026)
优惠价
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背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.

免疫原信息

免疫原Recombinant protein of human β-actin
序列Email for sequence
基因ID60
Swiss ProtP60709
别名ACTB; BRWS1; PS1TP5BP1; beta actin

应用

反应物种Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Rat , Clam , pig , Human , Chicken , Mouse , chinken , Zebrafish)

CoIP (Human)

WB (Human)

IHC (Mouse)

IHC (Mouse)

IF (Mouse)

推荐稀释比
WB 1:50000 - 1:200000
IHC 1:200 - 1:2000
IF 1:200 - 1:2000
抗体稀释方法Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.
理论分子量41kDa
实际分子量43kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本NIH/3T3,TE-1,293T,SW480,Mouse brain,Mouse testis,Mouse lung,Rat brain,HeLa
细胞定位Cytoplasm,cytoskeleton
纯化方式Affinity purification

ACTB Monoclonal Antibody 检测图片

ABclonal:Western blot - ACTB Monoclonal Antibody (AC026) }

Western blot - ACTB Monoclonal Antibody (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - ACTB Monoclonal Antibody (AC026) }

Western blot - ACTB Monoclonal Antibody (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC026) }

Immunofluorescence - ACTB Monoclonal Antibody (AC026)

Immunofluorescence analysis of HeLa cells using ACTB antibody (AC026) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

评论列表

CustomerAug 302018
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot (WB)
Sample
rat brain cells
Loading Amount
15 ug
Dilution
1:20000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Remarks
Western blot analysis of extracts of rat brain cells, using antibody at 1:50 dilution.

More

CustomerNov 042016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
Pig lung
Loading Amount
15 ug
Dilution
1:5000
Incubatition time
14h
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of Pig lung, using β-actin Monoclonal Antibody.

More

CustomerJul 092016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
293T
Loading Amount
25 ug
Dilution
1:50000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of 293T cells, using β-actin Monoclonal Antibody.

More

CustomerJul 082016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
DF-1
Loading Amount
0 ug
Dilution
1:50000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of DF-1 cells, using β-actin Monoclonal Antibody.

More

>

Reviews List

CustomerAug 302018
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot (WB)
Sample
rat brain cells
Loading Amount
15 ug
Dilution
1:20000
Incubatition time
over night
Temperature
4 ℃
Exposure
10s
Remarks
Western blot analysis of extracts of rat brain cells, using antibody at 1:50 dilution.

More

CustomerNov 042016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
Pig lung
Loading Amount
15 ug
Dilution
1:5000
Incubatition time
14h
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of Pig lung, using β-actin Monoclonal Antibody.

More

CustomerJul 092016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
293T
Loading Amount
25 ug
Dilution
1:50000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of 293T cells, using β-actin Monoclonal Antibody.

More

CustomerJul 082016
Review for ACTB Monoclonal Antibody(AC026)5
Cat.No
AC026
Application
Western blot(WB)
Sample
DF-1
Loading Amount
0 ug
Dilution
1:50000
Incubatition time
over night
Temperature
4 ℃
Exposure
20s
Observed Band
42 kDa
Remarks
Western blot analysis of extracts of DF-1 cells, using β-actin Monoclonal Antibody.

More

>

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