PYCARD Knockout HeLa Cell Line, Homozygous (RM02207)

Genome sequence analysis of PCR products from parental (WT) and PYCARD knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02207

详细信息

靶点

PYCARD

细胞系

HeLa

突变描述

PYCARD Knockout cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:20bp deletion in exon1
Allele-2:110bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell line and 1 vial knockout cell line

细胞数目

1~5x10⁶ cells/vial

使用方法

Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition.
1. Thaw the vial in 37℃ water bath ,and shake it to melt as soon as possible.
2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me-
dium and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3. Remove and discard the supernatant.
4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish.
5. Add 8-10mL of complete medium.
6. Incubate the culture at 37℃ incubator with 5% CO₂.
7. A subcultivation ratio of 1:2-1:4 is recommended.

物种

Human

保存条件

Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.

运输条件

Dry ice

背景信息

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

基因ID

29108

Swiss Prot

Q9ULZ3

别名

ASC; CARD5; TMS; TMS-1; TMS1

研究领域