产品中心 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

MAP1LC3A Knockout 293T Cell Lysate, Homozygous (RM50108)

说明书 联系我们 收藏

Genome sequence analysis of PCR products from parental (WT) and MAP1LC3A knockout (KO) 293T cells, using sanger sequencing.

All(1)|
货号: RM50108
促销价：   ￥4700
货    期：现货产品
抗体定制服务咨询

文献引用 (0)

详细信息

靶点
MAP1LC3A（293T北纳）
细胞系
293T
突变描述
MAP1LC3A Knockout cell line is engineered from 293T cell line with Gene-Editing Technology.
Allele-1:exon2 was deleted
Allele-2:exon2 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

基因ID
84557
Swiss Prot
Q9H492
别名
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A
研究领域
    >