ACKR3 Knockout 4T1 Cell Lysate, Homozygous (RM50146)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and ACKR3 knockout (KO) 4T1 cells, using sanger sequencing.

All(1)|

货号: RM50146

详细信息

靶点

ACKR3

细胞系

4T1

突变描述

ACKR3 Knockout cell line is engineered from 4T1 cell line with Gene-Editing Technology.
Allele-1:88bp deletion in exon1
Allele-2:1bp insertion and 3bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Mouse

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

Predicted to enable chemokine binding activity; chemokine receptor activity; and scavenger receptor activity. Acts upstream of or within negative regulation of cell population proliferation and positive regulation of ERK1 and ERK2 cascade. Predicted to be located in several cellular components, including cell surface; clathrin-coated pit; and endosome. Predicted to be active in external side of plasma membrane. Is expressed in several structures, including adrenal gland; central nervous system; genitourinary system; heart; and hemolymphoid system. Orthologous to human ACKR3 (atypical chemokine receptor 3).

基因ID

12778

Swiss Prot

P56485

别名

Rdc1; Cxcr7; RDC-1; CXC-R7; CXCR-7; Cmkor1