IMPDH1 Knockout NIH/3T3 cell lysate, Homozygous (RM50178)

Genome sequence analysis of PCR products from parental (WT) and IMPDH1 knockout (KO) NIH/3T3 cells, using sanger sequencing.

All(1)|

货号: RM50178

详细信息

靶点

IMPDH1

细胞系

NIH/3T3

突变描述

IMPDH1 Knockout cell line is engineered from NIH/3T3 cell line with Gene-Editing Technology.
Allele-1:67bp deletion in exon1
Allele-2:67bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Mouse

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

Enables IMP dehydrogenase activity. Involved in 'de novo' XMP biosynthetic process and GMP biosynthetic process. Acts upstream of or within lymphocyte proliferation and purine nucleotide biosynthetic process. Predicted to be located in cytosol and nucleus. Predicted to be active in cytoplasm. Is expressed in central nervous system and retina outer nuclear layer. Human ortholog(s) of this gene implicated in Leber congenital amaurosis 11; retinitis pigmentosa; and retinitis pigmentosa 10. Orthologous to human IMPDH1 (inosine monophosphate dehydrogenase 1).

基因ID

23917

Swiss Prot

P20839

别名

IMPDH-I; B930086D20Rik