TNFRSF18 Knockdown HeLa cell lysate, Heterozygous (RM50182)

Genome sequence analysis of PCR products from parental (WT) and TNFRSF18 knockdown (KD) HeLa cells, using sanger sequencing.

All(1)|

货号: RM50182

详细信息

靶点

TNFRSF18

细胞系

HeLa

突变描述

TNFRSF18 Knockdown cell line is engineered from HeLa cell line with Gene-Editing Technology.
Allele-1:130bp deletion in exon1
Allele-2:WT

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

基因ID

8784

Swiss Prot

Q9Y5U5

别名

AITR; GITR; CD357; GITR-D; ENERGEN; GITR/TNFRSF18

研究领域