产品和服务 / 产品类型 / ELISA试剂盒 / ELISA 抗体对
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 4 days, the stability of the standard curve all conform to CV <10%.
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
* This package insert must be read in its entirety before using this product.
Human TNF alpha ELISA assay
| Recommended Concentration | Sample | ||
|---|---|---|---|
| ELISA assay | Capture | 1-4ug/mL | Rabbit anti-Human TNF alpha mAb(Cat. No.RMK0415) |
| Detection | 0.125-0.5ug/mL | Rabbit anti-Human TNF alpha mAb(Cat. No. RMK0416) | |
| Standard | 0.78-50pg/mL | RecombinantHuman TNF alpha Protein (Cat. No. RP00001) | |
| Multiplex | Capture | 0.1-5ug/mL | Rabbit anti-Human TNF-alpha mAb(Cat. No. RMK0416) |
| Detection | 0.5-2ug/mL | Rabbit anti-Human TNF-alpha mAb (Cat. No. RMK0436) | |
| Standard | 6.86-5000pg/mL | Recombinant Human TNF-alpha Protein (Cat. No. RP00001) |
Tumour Necrosis Factor alpha (TNF alpha) is a homotrimer protein consisting of 157 amino acids, is a potent pro-inflammatory cytokine. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
