Recombinant Human IL-1 beta Protein (RP00002)

Recombinant Human IL-1 beta Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18 kDa.

Recombinant Human IL1β protein was measured by NF-κB reporter assay in HEK293 cells. The ED₅₀ for this effect is 1-10 pg/mL, corresponding to a specific activity of 1×10⁸~1×10⁹ units/mg.

Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.

Recombinant Human IL-1 beta promotes the proliferation of D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 6.04-24.16 pg/mL, corresponding to a specific activity of 4.14×10⁷~1.66×10⁸ units/mg.

All(4)|

货号: RP00002

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

18.22 kDa

Observed MW

18 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Recombinant Human IL1β protein was measured by NF-κB reporter assay in HEK293 cells. The ED₅₀ for this effect is 1-10 pg/mL, corresponding to a specific activity of 1×10⁸~1×10⁹ units/mg.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 0.217-0.870 ng/mL, corresponding to a specific activity of 1.15×10⁶~4.61×10⁶ units/mg.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (2)

折叠内容

CustomerNov 202024

评分
Experiment Type: Cell-based assay
Sample: MLE-12
Description: The cytokine combination (IFN-γ: 400ng/ml, Cat.RP01038; TNF-α: 400ng/ml, Cat.RP00993; IL-1β: 5ng/ml, Cat.RP00002) was dissolved into 10X cytomix using PBS. Then use the gradient dilution of 10 X cytomix stimulate C2C12 cells for 24 h, concentration as follows (0.25X,0.5X,1X,2X,4X), induction of cell damage, finally determined by MTT were used to detect cell damage

CustomerOct 232024

评分
Experiment Type: Cell-based assay
Sample: chondrocytes
Description: Human chondrocytes treated with 25 ng/mL IL-1β for 24 h showed increased expressions of type I collagen (COL I) and type X collagen (COL X), and decreased expressions of type II collagen (COL II) and glycosaminoglycan (ACAN), as expected.

背景信息

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

基因ID

3553

Swiss Prot

P01584

别名

IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B

研究领域