Recombinant Human RBBP-4 Protein (RP00029)

Recombinant Human RBBP-4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 50 kDa.

Immobilized recombinant Human RBBP4 Protein at 1μg/mL (100 μL/well) can bind RBBP4 Rabbit pAb with a linear range of 0.976-7.09ng/mL.

All(2)|

货号: RP00029

详细信息

种属

Human

表达宿主

Baculovirus-Infected Sf9 Cells

Calculated MW

47.66 kDa

Observed MW

50 kDa

标签

No tag

纯度

> 92% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 500mM NaCl, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human RBBP-4 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Met1-Ser425) of human RBBP-4 (Accession #NP_005601.1).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human RBBP4 Protein at 1 μg/mL (100 μL/well) can bind RBBP4 Rabbit pAb with a linear range of 0.976-7.09 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family.It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

基因ID

5928

Swiss Prot

Q09028

别名

NURF55; RBAP48; lin-53;RBBP4;RBAP48;lin-53

研究领域