Recombinant Human TNFRSF6/FAS/CD95 Protein (RP00139)

Recombinant Human FAS/APO-1/CD95 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 50-70 kDa.

Immobilized recombinant Human Fas Ligand at 2μg/mL (100 μL/well) can bind recombinant Human FAS, the EC₅₀ of Human FAS is 6.23 ng/mL.

Recombinant Human FAS inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells. The ED₅₀ for this effect is typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.

All(3)|

货号: RP00139

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

43.43 kDa

Observed MW

55-70 kDa

标签

C-hFc&His

纯度

> 90% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human TNFRSF6/FAS/CD95 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Asn173) of human FAS/CD95/APO-1/TNFRSF6 (Accession #NP_000034.1) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Fas Ligand at 2 μg/mL (100 μL/well) can bind recombinant Human FAS. The EC₅₀ of Human FAS is 6.23 ng/mL.
2.Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells. The ED₅₀ for this effect is typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The protein is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

基因ID

355

Swiss Prot

P25445

别名

ALPS1A;APO-1;APT1;CD95;FAS1;FASTM;TNFRSF6;FAS; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; Fas cell surface death receptor

研究领域