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Recombinant Mouse IL-4 Protein (RP01161)

文献引用 (8) 评论 (9) 说明书 收藏

Recombinant Mouse IL-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Mouse IL-4 Protein is greater than 95% as determined by SEC-HPLC.

Recombinant Mouse IL-4 promote the proliferation of MC/9 2 mouse mast cells. The ED50 for this effect is 2.8-11.3 pg/mL, corresponding to a specific activity of 8.85×107~3.57×108 units/mg.

Recombinant Mouse IL4 stimulates cell proliferation of HT-2 mouse T cells. The ED50 for this effect is 1.18-4.70 ng/mL, corresponding to a specific activity of 2.13×105~8.51×106 units/mg.

All(4)|
货号: RP01161

促销价：   ￥780
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
14.15 kDa
Observed MW
20 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Mouse IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His23-Ser140) of mouse IL-4 (Accession #NP_067258.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured in a cell proliferation assay using MC/9 2 mouse mast cells. The ED50 for this effect is 2.8-11.3 pg/mL, corresponding to a specific activity of 8.85×107~3.57×108 units/mg.
2.Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.21-0.85 ng/mL, corresponding to a specific activity of 1.18×106~4.76×106 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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客户数据及评论 (9)

折叠内容
中国人民解放军陆军军医大学第一附属医院Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
1、Extract the bone marrow cells of a 8-week-old male C57 mouse, stimulate the cells with mouse CSF-1 (Cat. RP01216) at a final concentration of 20 ng/mL for one week (change the culture medium every day). Collect the cells, and detect the result of macrophage induction by flow cytometry. 2、Stimulate the M0 cells with mouse IL-4 (Cat. RP01161) at a final concentration of 40 ng/mL. Then collect the cells detect the result of M2 polarization by flow cytometry.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Recombinant mouse IL-4 (20 ng/mL，Cat. RP01161) was used to induce the polarization of primary mouse bone marrow derived macrophages (BMDM) to M2 type for 24 h. Q-PCR result showed that the expression level of CD206 was about 20 times higher, and other indicators were about 2-3 times higher.
中国科学院上海药物研究所Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
In vitro induction culture of mouse bone marrow derived dendritic cells (BMDC): mouse bone marrow monocytes were treated with GM-CSF (20 ng/mL, Cat. RP01206) and IL-4 (10 ng/mL, Cat. RP01161) to differentiated into BMDC. The expression of CD11c and MHC Ⅱ were detected by flow cytometry.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
In vitro induction culture of mouse bone marrow derived dendritic cells (BMDC): C57BL/6 mouse bone marrow cells from femur and tibia were induced with IL-4 (10 ng/mL, Cat. RP01161) and GM-CSF (20 ng/mL, Cat. RP01206) to differentiate into BMDC. DCs were labeled with CD11c, and DCs maturation was detected by CD80 and CD86 at 7 days after induction. Flow cytometry result showed that the proportion of cells expressed CD11c was as high as 89.4%, and more than half of the cells expressed CD80 and CD86, indicating BMDC was successfully induced.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
C57BL/6 mouse bone marrow cells were induced with IL-4 (10 ng/mL, Cat. RP01161) and GM-CSF (20 ng/mL) to differentiate into bone marrow derived dendritic cells (BMDC). At 5 days and 6 days after induction, the expression of CD11c and MHC Ⅱ were detected by flow cytometry to analyze the maturation of BMDC.
中国科学院上海药物研究所Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
200 ng/mL of recombinant mouse IL-4 （Cat. RP01161） and IL-13（Cat. RP02112）were used to induce the polarization of primary mouse bone marrow derived macrophages (BMDM) to M2 type, which is characterized by high expression of Arg-1, Ym1, CD206 and other molecules. Detection result of Arg-1 expression level suggested that M2 macrophages were induced successfully.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
B cell
Description
Recombinant mouse IL-4 (1 ng/mL, Cat. RP01161) was used to culture B cells. After 4 days, there was obvious cluster-like cells, indicating that IL-4 provided necessary signal for the proliferation and activation of B cells.
中国科学院上海药物研究所Nov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Recombinant mouse IL-4 （Cat. RP01161） and IL-13（Cat. RP01123） were used to induce the polarization of primary mouse bone marrow derived macrophages (BMDM) to M2 type, which is characterized by high expression of Arg-1, Ym1, CD206 and other molecules. Detection result of Arg-1 expression level suggested that M2 macrophages were induced successfully.
浙江省人民医院Oct 232024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
On the sixth day of culture, mouse macrophages were added with: IL-10 (10 ng/mL, Cat. RP01465) + IL-4 (20 ng/mL, Cat. RP01161) to induce M2 macrophage polarization, and IFN-γ (50 ng /mL, Cat. RP01070) + LPS induced the polarization of M1 macrophages for two days. The changes in relevant markers were detected by flow cytometry, and the results showed that the induction was successful.

文献引用 (8)

Publishing research using RP01161? Please let us know so that we can cite the reference in this datasheet.

背景信息

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.

基因ID
16189
Swiss Prot
P07750
别名
Il-4; BSF-1;IL4