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活性验证

Recombinant Mouse CSF-1/M-CSF Protein (RP01216)

文献引用 (6) 评论 (8) 说明书 收藏

Active Recombinant Mouse CSF-1/M-CSF Protein was determined by SDS-PAGE with Coomassie Blue

Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng/mL, corresponding to a specific activity of 3.32×104~1.33×105 units/mg.

Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 12.5×104~5.0×105 units/mg.

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货号: RP01216

促销价：   ￥1100
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
30.19 kDa
Observed MW
40-55 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Mouse CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu262) of mouse M-CSF/CSF-1 (Accession #NP_031804.3.) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×106-1.0×107units/mg.
2.Measured in a cell proliferation assay using mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng/mL, corresponding to a specific activity of 3.32×104~1.33×105 units/mg.
3.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 12.5×104~5.0×105 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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客户数据及评论 (8)

折叠内容
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
20 ng/mL of recombinant mouse M-CSF (Cat. RP01216) was used to induce mouse primary bone marrow cells differentiate into macrophages (BMDM). After 7 days, the cells were in a normal state and morphology, indicating an effective induction.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Recombinant mouse M-CSF (10 ng/mL, Cat. RP01216) from ABclonal and P Brand were used to induce mouse bone marrow cells differentiate into macrophages respectively. The cell state and morphology showed that the induction effect was almost the same.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Mouse bone marrow cells were seeded in 12-well plates at a concentration of 1.5 million/mL, and 1 mL per well. The medium was high glucose DMEM supplemented with 10% FBS, 2‰ PS and 20 ng/mL of recombinant mouse M-CSF (Cat. RP01216) . This medium was changed every 2-3 days, and BMDM were obtained after 7 days of differentiation. For further validation, western-blot was used to detect the IL-1β produced by BMDM after stimulation with LPS.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Induce BMMs to differentiation into osteoclasts(Customer Feedback protocol): 1. Mouse bone marrow cells were obtained from long bones of C57BL/6J mice. 2. The cells were cultured in α-MEM medium supplemented with 25 ng/mL M-CSF (Cat. RP01216) in 10 cm cell culture dishes. 3. When the cell confluency reached about 90%, the cells (BMMs) were detached and plated in 96-well plates at 8000 cells/well. 4. Different concentration of RANKL (Cat. RP00745) and 25 ng/mL of M-CSF were supplemented to stimulate the BMMs. 5. Replaced with fresh medium every two days, and sitmulated the BMMs for three times in total. 6.After cell fixation, TRAP staining was performed
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
1、Extract the bone marrow cells of a 8-week-old male C57 mouse, stimulate the cells with mouse CSF-1 (Cat. RP01216) at a final concentration of 20 ng/mL for one week (change the culture medium every day). Collect the cells, and detect the result of macrophage induction by flow cytometry. 2、Stimulate the M0 cells with mouse IL-4 (Cat. RP01161) at a final concentration of 40 ng/mL. Then collect the cells detect the result of M2 polarization by flow cytometry.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
50 ng/mL of recombinant mouse M-CSF (Cat. RP01216) was used to induce mouse primary bone marrow cells differentiate into macrophages (BMDM). After 5 days, the cells were in a normal state and morphology, indicating an effective induction.
CustomerNov 122024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
20 ng/mL of recombinant mouse M-CSF (Cat. RP01216) was used to induce mouse primary bone marrow cells differentiate into macrophages (BMDM). After 7 days, the signals of markers CD11b and F4/80 detected by flow cytometry were both increased, indicating a successfully induction.
CustomerOct 232024
评分
Experiment Type
Cell-based assay
Sample
BMM
Description
Mouse bone marrow cells were taken and cultured in a cell culture dish for 3 hours. Following this, the cells were transferred to a bacterial culture dish and cultured in medium containing 20 ng/mL M-CSF. The medium was changed every 3 days, and after 7 days, the cells were matured. Flow cytometry was employed to assess changes in macrophage markers, revealing a positive rate of macrophages as high as 98%. Additionally, results from phagocytosis experiments indicated that the induced macrophages exhibited strong phagocytic capability.

文献引用 (6)

折叠内容

Publishing research using RP01216? Please let us know so that we can cite the reference in this datasheet.

背景信息

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

基因ID
12977
Swiss Prot
P07141-1
别名
MCSF;M-CSF;CSF-1;Lanimostim;CSF1
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