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Recombinant Rat VEGF-A/VEGF164 Protein (RP01323)

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Recombinant Rat VEGF-A/VEGF164 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-32kDa.

Immobilized Recombinant Rat VEGF164 at 1 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.03-3.6 ng/mL.

Recombinant Rat VEGF-A promotes the proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 0.02-0.10 ng/mL.

All(3)|
货号: RP01323
促销价：   ￥620
货    期：现货产品
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详细信息

种属
Rat
表达宿主
HEK293 cells
描述
Recombinant Rat VEGF-A/VEGF164 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Rat VEGF164 at 1 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.03-3.6 ng/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.02-0.10 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Vascular endothelial growth factor A (VEGFA),also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

基因ID
83785
Swiss Prot
P16612-2
别名
MVCD1;VAS;vascular endothelial growth factor A;Vasculotropin;VEGF;VEGFA;VEGF-A;VEGFMGC70609;VPF;VEGFA
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