产品和服务 / 产品类型 / 重组蛋白 / 生物标志物 & CD分子

活性验证

Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein (RP01375)

说明书 收藏

Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-50kDa.

The purity of Mouse TNFR2/CD120b/TNFRSF1B Protein (Cat.RP01375) was greater than 90% as determined by SEC-HPLC.

Immobilized Mouse TNFRSF1B at 1μg/mL (100 μL/well) can bind Mouse TNF-alpha with a linear range of 0.64-317.13ng/mL.

Recombinant Mouse TNFRSF1B inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 2-8 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

All(4)|
货号: RP01375
促销价：   ￥620
货    期：现货产品
抗体定制服务咨询 |
扫码下单
享受积分

详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
26.17 kDa
Observed MW
35-55 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val23-Gly258) of mouse TNFR2/CD120b/TNFRSF1B (Accession #NP_035740.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFRSF1B at 1 μg/mL (100 μL/well) can bind Mouse TNF-alpha with a linear range of 0.64-317.13 ng/mL.
2.Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 2-8 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

客户数据及评论 (0)

折叠内容

背景信息

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

基因ID
21938
Swiss Prot
P25119
别名
TNFRSF1B;CD120b;TBPII;TNF-R-II;TNF-R75;TNFBR;TNFR1B;TNFR2;TNFR80;p75;TNFRSF1B;TNFRSF1B;CD120b;TBPII;TNF-R-II;TNF-R75;TNFBR;TNFR1B;TNFR2;TNFR80;p75;TNFRSF1B
>