Recombinant Human CDH3/P-cadherin Protein (RP01491)

说明书联系我们收藏

Recombinant Human CDH3/P-cadherin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80kDa.

All(1)|

货号: RP01491

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

60.86 kDa

Observed MW

70-80 kDa

标签

C-His

纯度

> 85% by SDS-PAGE.

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human CDH3/P-cadherin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp108-Gly654) of human CDH3/Cadherin-3 (Accession #NP_001784.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1×105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 50-60% will adhere after 1.5 hours at 37° C.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Cadherin-3 （also known as P-cadherin）is a classical cell-to-cell adhesion molecule with a homeostatic function in several normal tissues. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. In humans, cadherin-3 is only detected in a few organs. However, its overexpression is strongly associated with a poor prognosis in some solid tumors including breast, lung and pancreatic cancers.

基因ID

1001

Swiss Prot

P22223

别名

CDH3;CDHP;HJMD;PCAD

研究领域