Recombinant Human Pro-neuregulin-1/NRG1 (Beta1) Protein (RP01825)

Recombinant Human Pro-neuregulin-1/NRG1（Beta1） Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 10-15 kDa.

Recombinant Human Pro-neuregulin-1/NRG1 (Beta1) stimulates serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is typically 0.62-2.48 ng/mL, corresponding to a specific activity of 4.03×10⁵~1.61×10⁶ units/mg.

All(2)|

货号: RP01825

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

8.06 kDa

Observed MW

10-15 kDa

标签

No-Tag

纯度

> 92% by SDS-PAGE.

内毒素

< 1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Pro-neuregulin-1/NRG1（Beta1） Protein is produced by E. coli expression system. The target protein is expressed with sequence (Thr 176-Lys 246) of human Pro-neuregulin-1/NRG1（Beta1） (Accession #NP_039250.2) fused with no additional

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is typically 0.62-2.48 ng/mL, corresponding to a specific activity of 4.03×10⁵~1.61×10⁶ units/mg.

复溶

Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

neuregulin-1 (heregulin-1， NRG1) is a member of neuregulin family, which is comprised of four genes thatencode a large number of secreted or membrane-bound isoforms. All family members share an EGF-likedomain that interacts with the ErbB family of tyrosine kinase receptors. NRG1 isoforms can be classified intotype I, type II and type III isoforms. NRG1 directs ligand for ERBB3 and ERBB4 tyrosine kinase receptors,concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylationand activation of the ERBB receptors. NRG proteins show distinct spatial and temporal expression patterns andplay important roles during development of both the nervous system and the heart.

基因ID

3084

Swiss Prot

Q02297-6（Beta1）

别名

GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2;Pro-neuregulin-1;NRG1（Beta1）

研究领域