Recombinant Human/Mouse/Rat mature BMP-2 Protein (RP02510S)

Recombinant Human/Mouse/Rat mature BMP-2 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions ，showing single bands at 15-20 kDa and 25-30 kDa, respectively.

Recombinant Human/Mouse/Rat mature BMP-2 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED₅₀ for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×10⁴~4.07×10⁴ units/mg.

All(2)|

货号: RP02510S

详细信息

种属

Human

表达宿主

E.coli

Calculated MW

12.78 kDa

Observed MW

15-20 kDa

标签

No tag

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 30% ACN. Contact us for customized product form or formulation.

描述

Recombinant Human/Mouse/Rat mature BMP-2 Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence (Ala284-Arg396) of Human/Mouse/Rat BMP-2 (NP_001191.1) fused with no tag.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED₅₀ for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×10⁴~4.07×10⁴ units/mg.

复溶

Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (1)

折叠内容

CustomerNov 122024

评分
Experiment Type: Cell-based assay
Sample: MC3T3
Description: Hydrogel-loaded BMP-2 (Cat.RP02510S) promoted the osteogenic differentiation of mouse embryonic osteoblastic precursor cell line MC3T3. After 7 days, ALP staining was used to observe the cell differentiation, and the effect was significant.

背景信息

BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.

基因ID

650

Swiss Prot

P12643

别名

BDA2;BMP2A;BMP2

研究领域