Recombinant Human PARP1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The PARP1 activity was assayed with ELISA. The reaction was performed by:(1)Histone protein was coated on a 96-well plate. (2)A biotinylated NAD+ mix is incubated with varying different concentration of the PARP1 enzyme and an activated DNA. (3)The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
