Recombinant Human PARP1 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 115.1 kDa.
The PARP1 activity was assayed with ELISA. The reaction was performed by:(1)Histone protein was coated on a 96-well plate. (2)A biotinylated NAD+ mix is incubated with varying different concentration of the PARP1 enzyme and an activated DNA. (3)The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
Recombinant human PARP1 protein with N-terminal 6x his tag +TEV cleavage site was purified by Ni-NTA affinity and followed by SEC chromatography. The PARP1 protein showed high activity in ELISA assay
标签
N-6His
纯度
> 95% by SDS-PAGE
生物活性
PARP1 activity test using ELISA method.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Hepes, 300mM NaCl, 1mM DTT, pH7.0
储存
This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。