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活性验证

Recombinant Mouse FSTL3 Protein (RP03254)

Recombinant Mouse FSTL3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa.

The purity of Mouse FSTL3 is greater than 95% as determined by SEC-HPLC.

Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μL/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0 ng/mL determined by ELISA.

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.

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货号: RP03254
促销价:   ¥3200
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
26 kDa
Observed MW
35-40 kDa
标签
C-His
纯度
> 95% by Tris-Bis PAGE and HPLC.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
描述
Recombinant Mouse FSTL3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Val256) of Mouse FSTL3 (Accession #Q9EQC7) fused with His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
1. Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μL/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0 ng/mL determined by ELISA.
2. Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200).
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

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折叠内容

背景信息

Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.

基因ID
Swiss Prot
别名
Follistatin-like protein 3; FLRG; Follistatin-like 3; FSTL3
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