产品和服务 / 产品类型 / 重组蛋白 / 生物标志物 & CD分子

活性验证

Recombinant Mouse IL-6RB/gp130/CD130 Protein (RP03281)

Recombinant Mouse IL-6RB/gp130/CD130 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-90 kDa.

All(1)|
货号: RP03281
促销价:   ¥2250
货    期:在线咨询货期
抗体定制服务咨询 |
商城订购

详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
68 kDa
Observed MW
80-90 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
描述
Recombinant Mouse IL-6RB/gp130/CD130 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu617) of mouse IL-6RB/gp130/CD130 (Accession #Q00560) fused with His tag at the C-terminus.
储存
Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
=
÷

客户数据及评论 (0)

折叠内容

背景信息

gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis.

基因ID
Swiss Prot
别名
Interleukin-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130; CD130; Il6st; Oncostatin-M receptor subunit alpha; CDw130
>