Recombinant Human CDK1&Cyclin E1 Protein was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of CDK1/Cyclin E1 is based on the MSA technology, and the content and ratio of the substrate and the product are directly separated and detected in real time and dynamically by the different migration rates of the substrate and the product after the enzymatic reaction.
CDK1 also known as cyclin-dependent kinase 1 or cell division cycle protein 2 homolog is a highly conserved protein that functions as a serine/threonine protein kinase, and is a key player in cell cycle regulation. CDK1 is a small protein (approximately 34 kDa), and is highly conserved. When bound to its cyclin partners, CDK1 phosphorylation leads to cell cycle progression. Given its essential role in cell cycle progression, CDK1 is highly regulated. Most obviously, CDK1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of CDK1, allowing for CDK1 activity. Cyclin E1 functions as a regulator of S phase entry and progression in mammalian cells. It was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
