HK1 Knockout 293T Cell Lysate, Homozygous (RM01976)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and HK1 knockout (KO) 293T cells, using sanger sequencing.

All(1)|

货号: RM01976

详细信息

靶点

HK1

细胞系

293T

突变描述

HK1 Knockout 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:4bp deletion in exon2
Allele-2:4bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

基因ID

3098

Swiss Prot

P19367

别名

HK; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; hexokinase

研究领域