TUBB3 Knockdown HeLa Cell Lysate, Heterozygous (RM02073)

Genome sequence analysis of PCR products from parental (WT) and TUBB3 Knockdown (KD) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02073

详细信息

靶点

TUBB3

细胞系

HeLa

突变描述

TUBB3 Knockdown HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:120bp deletion and 31bp insertion in exon2
Allele-2:117bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

基因ID

10381

Swiss Prot

Q13509

别名

CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4; beta-4

研究领域