RB1 Knockout HeLa Cell Lysate, Homozygous (RM02077)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and RB1 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02077

详细信息

靶点

RB1

细胞系

HeLa

突变描述

RB1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:exon1 was deleted
Allele-2:exon1 was deleted

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

基因ID

5925

Swiss Prot

P06400

别名

OSRC; PPP1R130; RB; p105-Rb; pRb; pp110

研究领域