ARHGDIA Knockout 293T Cell Lysate, Homozygous (RM02373)

Genome sequence analysis of PCR products from parental (WT) and ARHGDIA knockout (KO) 293T cells, using sanger sequencing.

All(1)|

货号: RM02373

详细信息

靶点

ARHGDIA

细胞系

293T

突变描述

ARHGDIA Knockout 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:91bp deletion in exon1
Allele-2:91bp deletion in exon1

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

基因ID

396

Swiss Prot

P52565

别名

GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

研究领域