MLH1 Knockout HeLa Cell Lysate, Homozygous (RM02458)

说明书收藏

Genome sequence analysis of PCR products from parental (WT) and MLH1 knockout (KO) HeLa cells, using sanger sequencing.

All(1)|

货号: RM02458

详细信息

靶点

MLH1

细胞系

HeLa

突变描述

MLH1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:68bp deletion in exon3
Allele-2:68bp deletion in exon3

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

Sanger Sequencing

产品组成

1 vial parental cell Lysate and 1 vial knockout cell Lysate

裂解液量

50μL, 2μg/μL.

使用方法

To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.

物种

Human

保存条件

Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

运输条件

4℃

背景信息

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

基因ID

4292

Swiss Prot

P40692

别名

COCA2; FCC2; HNPCC; HNPCC2; hMLH1

研究领域