产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

YAP1 Knockout HeLa Cell Lysate, Homozygous (RM02599)

Genome sequence analysis of PCR products from parental (WT) and YAP1 knockout (KO) HeLa cells, using sanger sequencing.

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货号: RM02599
促销价:   ¥4700
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详细信息

靶点
YAP1
细胞系
HeLa
突变描述
YAP1 Knockout HeLa Cell Line is engineered from HeLa cell line with Gene-Editing technology.
Allele-1:34bp deletion in exon2
Allele-2:34bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

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