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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
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Mouse IL-6 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-2ug/mL | Rabbit anti-Mouse IL-6(CAP)(Cat. No.RM17709) |
| ELISA Detection | 0.0125-0.05ug/mL | Rabbit anti-Mouse IL-6(CAP)(Cat. No.RM17710) |
| Standard | 7.81-500pg/mL | Recombinant Mouse IL-6 Protein |
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates "classic signaling", the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates "trans-signaling". Alternatively, "cluster signaling" occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
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