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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
Fromthe comparison of the standard curve, it is found that ABclonal has highsensitivity.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Human VEGF165 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-2ug/mL | Rabbit anti-Human VEGF165 (CAP)(Cat. No.RM17780) |
| ELISA Detection | 0.005-0.02ug/mL | Rabbit anti-Human VEGF121 (DET)(Cat. No.RM17781) |
| Standard | 31.2-2000pg/mL | Recombinant Human VEGF165 Protein |
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
