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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
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Human EGFR Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Human EGFR mAb(CAP)(Cat. No.RM98010) |
| ELISA Detection | 0.0125-0.05ug/mL | Rabbit anti-Human EGFR mAb(DET)(RM98011) |
| Standard | 78.125-5000pg/mL | Recombinant Human EGFR Protein |
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades toconvert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha,amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand bindingtriggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. Thephosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signalingcascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT,PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylatesother proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to theG protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC andCTNNB1/beta-catenin
