产品和服务 / 产品类型 / ELISA试剂盒 / ELISA 抗体对
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
* This package insert must be read in its entirety before using this product.
Mouse/Rat IFN-gamma Multiplex
| Recommended Concentration | Sample | ||
|---|---|---|---|
| Multiplex | Capture | 3-20ug/mL | Rabbit anti-Mouse/Rat IFN-gamma mAb(Cat. No. RMK0001) |
| Detection | 0.017-2ug/mL | Rabbit anti-Rat IFN-gamma mAb(Cat. No.RMK0002) | |
| Standard | / | / | |
| Multiplex | Capture | 0.1-5ug/mL | Rabbit anti-Mouse IFN-gamma mAb(Cat. No. RMK0050) |
| Detection | 0.5-2ug/mL | Rabbit anti-Mouse/Rat IFN-gamma mAb (Cat. No. RMK0001) | |
| Standard | 6.86-5000pg/mL | Recombinant Mouse IFN-gamma Protein (Cat. No.RP01070) |
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine . Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits. It shares 86% amino acid sequence identity with mouse IFN-gamma and 37%?45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN? gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis .
