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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Human IL-1 beta Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4 ug/mL | Rabbit anti-Human IL-1 beta (CAP)(Cat. No.RMK0033) |
| ELISA Detection | 0.125-0.5ug/mL | Rabbit anti-Human IL-1 beta (DET)(Cat. No.RMK0034) |
| Standard | 0.625-40pg/mL | Recombinant Human IL-1 beta Protein |
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
