Recombinant Human MMP-13 Protein (RP00221)

Recombinant Human MMP-13 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 58 kDa.

Recombinant Human MMP-13 induces cell migration of the A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.

All(2)|

货号: RP00221

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

52.52 kDa

Observed MW

58 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human MMP-13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Cys471) of human MMP-13 (Accession #NP_002418.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell migration assay using A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >2900 pmol/min/μg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases ,it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.

基因ID

4322

Swiss Prot

P45452

别名

CLG3; MANDP1; MDST; MMP-13;MMP13;MANDP1;MDST;MMP-13

研究领域