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Biotinylated Recombinant Human B7-2/CD86 Protein (RP00561B)

说明书 收藏

Biotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Immobilized Biotinylated Human B7-2, His Tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.9ng/mL determined by ELISA.

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货号: RP00561B
促销价：   ￥5600
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
28.2 kDa
Observed MW
55-70 kDa
标签
C-His&Avi
纯度
> 95% by SDS-PAGE
内毒素
< 1 EU/μg of the protein by LAL method
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
描述
Biotinylated Recombinant Human B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu26-Pro247) of Human B7-2/CD86 (Accession #P42081-1) fused with a C-His&Avi tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

基因ID
942
Swiss Prot
P42081-1
别名
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
研究领域

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