Recombinant Human NKG2-2A/KLRC1/CD159a Protein (RP00571)

Recombinant Human NKG2-2A/KLRC1/CD159a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

All(1)|

货号: RP00571

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

16.5 kDa

Observed MW

25-40 kDa

标签

N-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.

描述

Recombinant Human NKG2-2A/KLRC1/CD159a Protein is produced by Human cells expression system. The target protein is expressed with sequence (Arg100-Leu233) of human NKG2-A/NKG2-B Type II Integral Membrane Protein (Accession #P26715) fused with an 8×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

NKG2-A/NKG2-B Type II Integral Membrane Protein contains 1 C-type lectin domain and belongs to the killercell lectin-like receptor family. The killer cell lectin-like receptor family is a group of transmembrane proteinspreferentially expressed in NK cells. Members of this proteins is characterized by the type II membraneorientation and the presence of a C-type lectin domain. NKG2 is expressed only in NK-cells, but not in T-cells orB-cells. It has been shown that NKG2 represents a family of related cDNA clones, designated NKG2A, NKG2B,NKG2C, and NKG2D, which encode type 2 integral membrane proteins (extracellular C-terminus) containing aC-type lectin domain. NKG2 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NKcells and some cytotoxic T-cells. NKG2A and NKG2B have been given the designation CD159a in thenomenclature of CD antigens.

基因ID

3821

Swiss Prot

P26715

别名

KLRC1;CD159A;NKG2;NKG2A

研究领域