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活性验证

Recombinant MERS-CoV Spike S1 Protein (RP01303)

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Recombinant MERS-CoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 120-150 kDa.

Immobilized Human CD26 (Catalog: RP01300LQ) at 2μg/mL (100μL/well) can bind MERS-CoV Spike S1 with a linear range of 0.5-420.21ng/mL.

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货号: RP01303
促销价：   ￥780
货    期：现货产品
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详细信息

种属
MERS-CoV
表达宿主
HEK293 cells
Calculated MW
104.08 kDa
Observed MW
130-150 kDa
标签
C-mFc
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant MERS-CoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr18-Glu725) of mers-cov Spike S1 (Accession #YP_009047204.1) fused with a mFc tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human CD26 (Catalog: RP01300LQ) at 2μg/mL (100μL/well) can bind MERS-CoV Spike S1 with a linear range of 0.5-420.21ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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文献引用 (2)

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Publishing research using RP01303? Please let us know so that we can cite the reference in this datasheet.

背景信息

The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

基因ID
14254594
Swiss Prot
K9N5Q8
别名
MERS-CoV Spike RBD
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