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活性验证

Recombinant Mouse IL-17A/CTLA-8 Protein (RP01460)

Recombinant Mouse IL-17A/CTLA-8 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 16-24kDa.

Immobilized Mouse IL-17A/CTLA-8 at 2 μg/mL (100 μL/well) can bind Mouse IL-17RA/CD217 with a linear range of 0.1-26 ng/mL.

Recombinant Mouse IL-17A/CTLA-8 Protein induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(Catalog: RP01071).The ED50 for this effect is 0.26-1.04 ng/mL, corresponding to a specific activity of 9.62×105~3.85×106 units/mg.

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货号: RP01460

促销价:   ¥800
货    期:现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
15.82 kDa
Observed MW
16-24 kDa
标签
C-His
纯度
> 90% by SDS-PAGE.
内毒素
<0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse IL-17A/CTLA-8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala26-Ala158) of mouse IL-17A/CTLA-8 (Accession #NP_034682.1.) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-17A/CTLA-8 at 2 μg/mL (100 μL/well) can bind Mouse IL-17RA/CD217 with a linear range of 0.1-26 ng/mL.Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(Catalog: RP01071).The ED50 for this effect is 0.26-1.04 ng/mL, corresponding to a specific activity of 9.62×105~3.85×106 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.

基因ID
Swiss Prot
别名
Il17;Ctla8;IL-17;Ctla-8;IL17A;IL-17A