Recombinant Mouse IL-7 Protein (RP01766LQ)

Recombinant Mouse IL-7 Proteinwas determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 kDa.

Recombinant Mouse IL-7 stimulates cell proliferation of the murine 2E8 cells. The ED₅₀ for this effect is 0.8-3.2 ng/mL, corresponding to a specific activity of 3.23×10⁵~1.25×10⁶ units/mg.

All(2)|

货号: RP01766LQ

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

15.74 kDa

Observed MW

20-30 kDa

标签

C-6His

内毒素

< 0.01EU/μg

制剂

Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

描述

Recombinant Mouse IL-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu26-Ile154) of mouse IL7(Accession #NP_032397.1) fused with and a 6×His tag at the C-terminus.

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using murine 2E8 cells. The ED₅₀ for this effect is 0.8-3.2 ng/mL, corresponding to a specific activity of 3.23×10⁵~1.25×10⁶ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCR?) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

基因ID

16196

Swiss Prot

P10168

别名

Il-7; hlb368; A630026I06Rik；il7