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活性验证

Recombinant Rat IL-4 Protein (RP01874)

说明书 收藏

Recombinant Rat IL-4 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 kDa.

Recombinant Rat IL-4 stimulates cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×102~1.54×103 units/mg.

All(2)|
货号: RP01874

促销价：   ￥1100
货    期：现货产品
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详细信息

种属
Rat
表达宿主
HEK293 cells
Calculated MW
14.60 kDa
Observed MW
15-25 kDa
标签
C-His
内毒素
<0.01EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Rat IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys25-Ser147) of Rat IL-4 (Accession #NP_958427.1) fused with a His at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×102~1.54×103 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

基因ID
287287
Swiss Prot
P20096
别名
B-cell growth factor 1; B-cell IGG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
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